November 13-19, 2022

Ljubljana, Slovenia

Electroporation-Based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

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Preliminary programme:

Electrical properties of cells and tissues in electric field

  • Resting and induced transmembrane voltage
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  • Dielectric properties of biological tissues
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  • Temperature and conductivity changes in tissues
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    Physical chemistry of membrane electroporation

  • Electrochemical membrane thermodynamics
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  • Molecular dynamics simulations
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    Electroporation in vitro and in vivo - protocols

  • Electroporation of cells in vitro
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  • Electroporation of cells in vivo
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    Development of devices and electrodes

  • Devices for in vitro and in vivo experimental work
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  • Electrodes for in vitro and in vivo
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  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
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    Electrochemotherapy of tumors

  • Basic mechanisms of electrochemotherapy
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  • Preclinical studies
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  • Clinical use of electrochemotherapy
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  • Current developments and future perspectives
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    Application of electroporation in gene transfection

  • Gene transfection in vitro
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  • Gene transfection in vivo
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  • Electroporation for gene therapy
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  • Electroporation for DNA vaccine
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    Highlights of 2022:
  • Nanosecond strobe fluorescence confocal micrography of cell transmembrane voltage, Joel N. Bixler
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  • Pulsed Field Ablation (PFA): current knowledge and future challenges, Tomás García-Sánchez
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  • IRE in Interventional Radiology, Martijn Meijerink
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  • Membrane repair mechanismis, Jesper Nylandsted
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  • Novel processing technologies in food processing, Javier Raso
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  • Treatment of Epilepsy with Temporal Interference Stimulation and Focal Ablations using Electroporation, Adam Williamson
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  • DNA electrophoresis is gene electro transfer, Shaurya Sachdev
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    Laboratory and practical work

  • The influence of Mg2+ ions on gene electotransfer efficiency
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  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
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  • Electropermeabilization detection with propidium iodide in plated cells
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  • Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
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  • Electrotransformation of Escherichia coli with plasmid DNA
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  • E. coli inactivation in a continuous flow system
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  • Electroporation of planar lipid bilayers
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  • Analysis of electric field orentations on gene electrotransfer visualization at the membrane level
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  • Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
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  • Numerical Modeling of Thermal Effects during Irreversible Electroporation Treatments
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  • Molecular dynamics simulations of membrane electroporation
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  • Electroporation of cells and tissues - interactive e-learning
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  • Measurement of electroporation pules with osciloscope; voltage and current probes
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  • Development of pulse power generators for electroporation
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